Chiyo first appeared in the Grade C Mission "Grandmother's Gift". In the Grandmother's Gift, it is her granddaughter's birthday and she reserved a Pearl Kunai from a jeweler for her granddaughter. Since Chiyo didn't have enough time to get the Pearl Kunai for Aiko since she wanted to make an ice-frosted cake for Aiko, so the main character helped Chiyo to get the Pearl Kunai for her and as a reward, Chiyo reserved a piece of cake for the character.
- Level 40 - Jounin Exam 4/5: The Elements
- Level 6 - Grandmother's Gift (first appearance)
Mother's Day 2009
- Level 8 - Happy Mother's Day
Chiyo could be based off of Pinako Rockbell from Full Metal Alchemist.